Saif Ali
BS Biotechnology student at IIUI, building a bridge between wet-lab genomics and computational biology — from primer design to Python-driven analysis.
The person behind the pipette and the terminal
I'm a BS Biotechnology student at the International Islamic University, Islamabad, driven by a desire to integrate computational tools with biological research. Most of my current focus sits at the intersection of bioinformatics and computational genomics — using data to ask better biological questions.
I've picked up hands-on experience at NIGAB-NARC, PCSIR, and the NIH Pakistan, working across DNA extraction, gel electrophoresis, PCR and primer design, while building parallel comfort with Python and R for biological data.
Research interests
Education
BS Biotechnology
International Islamic University, Islamabad · Sept 2023 – Sept 2027 (expected)FSc Pre-Medical
Higher secondary educationMatriculation
Secondary schoolContact
- saifali15874@gmail.com
- linkedin.com/in/saif-ali-421b20369
- Jhelum, Punjab, Pakistan
Internships & lab rotations
Three placements across genomics, health sciences and multi-disciplinary lab work.
- Designed and validated PCR primers for the OsARF11 gene using Primer-BLAST and NCBI databases.
- Ran gel electrophoresis to confirm amplification products and assess primer specificity.
- Performed DNA extraction and PCR optimization across multiple rice genotype samples.
- Documented protocols and results for genomics workflows under supervisor review.
- Rotated through health sciences laboratories, observing diagnostic and research workflows.
- Assisted with sample handling and basic lab documentation practices.
- Gained exposure to public-health-oriented lab operations and quality standards.
- Rotated across multiple analytical and microbiology labs over an 8-week placement.
- Practiced titration, NanoDrop quantification, and general instrumental analysis techniques.
- Learned media preparation and core microbiology handling protocols.
- Compiled a multi-lab techniques report summarizing methods and findings.
Reports, studies & ongoing work
Click a card to flip it and read the details.
Dengue Fever Awareness Survey
Community-level survey assessing public knowledge and prevention practices around dengue fever.
Click to flipDesigned and distributed a structured questionnaire to evaluate awareness of dengue transmission, symptoms and prevention within the local community, then analyzed responses to identify knowledge gaps.
Cas12a (Cpf1) Nuclease Study
Literature-based study on the structure, mechanism and applications of the Cas12a nuclease in genome editing.
Click to flipReviewed how Cas12a's single RNA-guided nuclease mechanism differs from Cas9, its staggered-cut DNA repair implications, and its growing role in diagnostics and genome engineering.
PCSIR Lab Techniques Report
Consolidated report covering the analytical and microbiology techniques practiced during the PCSIR rotation.
Click to flipDocumented titration procedures, NanoDrop quantification, media preparation and instrumentation basics across the 8-week, multi-lab PCSIR rotation.
NIGAB Genomics Report
Summary report of primer design and PCR-based genomics work carried out at NIGAB-NARC.
Click to flipDetailed the primer design workflow for OsARF11 using Primer-BLAST, the PCR optimization steps taken, and gel electrophoresis results confirming amplification.
ML in Bioinformatics
Exploring machine learning approaches applied to biological and genomic datasets.
Click to flipCurrently studying how classification and clustering methods can be applied to genomic and expression data as a foundation for future computational biology work.
R Programming for Biological Data
Building fluency in R for statistical analysis and visualization of biological datasets.
Click to flipWorking through R fundamentals — data wrangling, statistical tests and plotting — with a focus on applying them to omics-style datasets.
Lab, computational & analytical toolkit
Lab Techniques
Bioinformatics
Programming
Analytical
Skill overview
Let's talk research, internships or collaboration
Direct contact
- saifali15874@gmail.com
- Jhelum, Punjab, Pakistan